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his tag imidazole

his tag imidazole

Imidazole and pH gradient are the two most common strategies used in the Ni-NTA purification of his-tagged recombinant proteins. Imidazole is the competitive inhibitor for histidine-tagged protein, while the pH gradient offers elution of protonated his-ta

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his tag imidazole 相關資訊

  • Imidazole and pH gradient are the two most common strategies used in the Ni-NTA purificati...
    ... elution during Ni-NTA affinity purification, imidazole ...
    https://www.researchgate.net
  • His Tag Purification Purification Protocol ! Theory’andIntroduction:Ni1Affinity’Chromatogr...
    2015 His tag Purification Protocol - University of San Diego ...
    http://home.sandiego.edu
  • The imidazole side chains of a His-tag can engage inreversible coordinative bonds to certa...
    His-Tag - Roche Life Science | Welcome
    https://lifescience.roche.com
  • Imidazole competes with the his-tag for binding to the metal-charged resin and thus is use...
    His-Tag Purification | Chromatography Media, Columns & ...
    http://www.bio-rad.com
  • The result is expression of a recombinant protein with a 6xHis or poly-His-tag fused to it...
    His-tagged Proteins–Production and Purification | Thermo ...
    https://www.thermofisher.com
  • An excess of imidazole is passed through the column, which displaces the His-tag from nick...
    Imidazole - Wikipedia
    https://en.wikipedia.org
  • Imidazole has traditionally been added to the wash buffer to modulate non-specific binding...
    Imidazole Products | Sigma-Aldrich
    http://www.sigmaaldrich.com
  • A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histi...
    Polyhistidine-tag - Wikipedia
    https://en.wikipedia.org
  • purification of His-tagged protein, while WEPRO1240 is a standard type of wheat germ extra...
    Purification of His-tagged protein - CellFree Sciences ...
    http://www.cfsciences.com
  • I have tried to elute His tag protein (37AA) from the Ni column by 200mM imidazole buffer ...
    Why hasn't His Tag protein eluted from Nickel column? ...
    https://www.researchgate.net